Oʻzbekcha
OROLBO‘YI EKSTREMAL EKOTIZIMLARIDAN OLINGAN MAGLARDA AZOT METABOLIZMI POTENSIALI: QIYOSIY GENOMIK TAHLIL
Jurnal
Zamonaviy fan: innovatsion yondashuvlar va yosh tadqiqotchilarning dolzarb izlanishlari
Nashr
Zamonaviy fan: innovatsion yondashuvlar va yosh tadqiqotchilarning dolzarb izlanishlari
Annotatsiya
Mazkur tadqiqotda Orolbo‘yi ekstremal ekotizimlaridan rekonstruksiya qilingan bakterial metagenomik yig‘ilgan genomlarda (MAG) azot almashinuvi bilan bog‘liq funksional genlarning tarqalishi qiyosiy genomik tahlil qilindi. Natijada atmosfera azotini fiksatsiyasi, nitratni qaytarish, DNRA hamda denitrifikatsiyaning ayrim bosqichlariga oid genlar faqat ayrim genomlarda aniqlanib, ularning notekis tarqalganligi kuzatildi. Shu bilan birga, to‘liq denitrifikatsiya (nosDZ), nitrifikatsiya va anammoks jarayonlariga oid genlar aniqlanmadi. Olingan natijalar Orolbo‘yi cho‘kindi mikrobiotasida azot almashinuvi asosan anaerob metabolik yo‘llar orqali amalga oshishini hamda ushbu jarayonlar mikroorganizmlar o‘rtasidagi funksional ixtisoslashuv asosida tashkil topganligini ko‘rsatadi
Kalit so‘zlar
DNRA
MAG
Orolbo‘yi
anammoks
azot almashinuvi
denitrifikatsiya
metagenomika
nitrifikatsiya
qiyosiy genomika
Русский
В настоящем исследовании проведён сравнительный геномный анализ функциональных генов азотного обмена в реконструированных бактериальных метагеномных геномах (MAG), полученных из экстремальных экосистем Приаралья. Установлено, что гены, связанные с фиксацией атмосферного азота, восстановлением нитрата, процессом DNRA и отдельными стадиями денитрификации, выявлены лишь в части исследованных геномов, что свидетельствует об их неравномерном распределении. При этом гены, ответственные за полную денитрификацию (nosDZ), нитрификацию и анаммокс, обнаружены не были. Полученные результаты показывают, что азотный обмен в донной микробиоте Приаралья осуществляется преимущественно посредством анаэробных метаболических путей и характеризуется функциональной специализацией микроорганизмов
DNRA
MAG
Приаралье
азотный обмен
анаммокс
денитрификация
метагеномика
нитрификация
сравнительная геномика
English
This study presents a comparative genomic analysis of nitrogen metabolism-related functional genes in bacterial metagenome-assembled genomes (MAGs) reconstructed from the extreme ecosystems of the Aral Sea region. Genes involved in atmospheric nitrogen fixation, nitrate reduction, dissimilatory nitrate reduction to ammonium (DNRA), and several steps of denitrification were detected only in a limited number of genomes, indicating their uneven distribution among the analyzed MAGs. In contrast, genes responsible for complete denitrification (nosDZ), nitrification, and anammox were not identified. These findings suggest that nitrogen cycling in the sediment microbiota of the Aral Sea region is mainly driven by anaerobic metabolic pathways and is based on functional specialization among different microbial populations
Aral Sea region
DNRA
anammox
comparative genomics
denitrification
metagenome-assembled genomes (MAGs)
metagenomics
nitrification
nitrogen metabolism
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